Centrifuge safety protection measures should be considered when purchasing
There are many types of centrifuges, usually classified from the following aspects:
(1) According to the size of the speed can be divided into: low speed centrifuge, high speed centrifuge and high speed centrifuge;
(2) According to the requirements of temperature can be divided into: ordinary centrifuge and frozen centrifuge;
(3) According to the different rotor is divided into: horizontal rotor centrifuge and Angle rotor centrifuge;
(4) According to the size of the centrifuge volume can also be divided into: floor centrifuge, desktop centrifuge, palm centrifuge, etc.;
(5) According to the grade of centrifuges, it can also be divided into ordinary centrifuges and fine centrifuges.
Ordinary centrifuges have few functions, and are not fine in terms of speed and capacity, such as: the speed of an ordinary centrifuge is 5000 RPM, but the actual speed can not reach 5000 RPM. Such centrifuges are only suitable for ordinary centrifugal work, such as separating serum and concentrating urine.
Fine centrifuge has many functions, fine speed and capacity, and is suitable for some experiments with high requirements for centrifuges, such as: PCR experiments, blood component separation (mostly used in blood stations).
Choose ordinary centrifuges, according to the size of the workload, mainly from two aspects of speed and capacity. The following details the purchase of fine centrifuge should pay attention to the problems:
(1) Speed: Centrifuges are divided into low-speed centrifuges (< 10,000 rpm/min), high-speed centrifuges (10,000 rpm/min a 30,000 rpm/min), and high-speed centrifuges (> 30,000 rpm/min) according to the speed, each centrifuge has a rated speed, and the speed refers to the speed under no-load conditions. However, the speed varies according to the type of rotor and the size of the sample mass.
For example, the rated speed of a centrifuge is 16000 rpm/min, indicating that the rotor rotates 16,000 times per minute when the load is not loaded, and the speed will certainly be less than 16000 rpm/min after adding the sample. The rotor is different, the speed is also different (an imported centrifuge can be selected with multiple rotors), the horizontal rotor can reach 1 5000 rpm/min, but the Angle rotor can reach about 14000 rpm/min, the specific difference to consult the product sales personnel and the relevant technical personnel of the production plant in detail, so the choice of speed should be careful, The selected centrifuge speed should be higher than the target speed. For example, if the target speed is 16000 rpm/mIn, the selected centrifuge speed must be higher than 16000 rpm/min.
(2) Temperature: Some samples (such as proteins, cells, etc.) will be destroyed in a high temperature environment, which requires the choice of frozen centrifuges, which have a rated temperature range. Centrifuge at high speed when the heat generated and centrifuge refrigeration system balance at a certain temperature (general frozen centrifuge samples need to be maintained at 3 ° C ~ 8 ° C), the specific amount can be achieved is also related to the rotor, such as a centrifuge rated temperature range of -10 ° C ~ 60 ° C, installed in the horizontal rotor can reach about 3 ° C when rotating, If it is an angular rotor, it may only reach about 7 ° C. This point should also consult the product sales personnel and the relevant technical personnel of the production plant in detail.
(3) Capacity: how many sample tubes need to be centrifuged each time, how much capacity each sample tube needs, these factors determine the total capacity of a centrifuge, simply speaking, the total capacity of the centrifuge.
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